Middle East Respiratory Syndrome Coronavirus Intra-Host Populations Are Characterized by Numerous High Frequency Variants.
Identifieur interne : 000F89 ( Main/Exploration ); précédent : 000F88; suivant : 000F90Middle East Respiratory Syndrome Coronavirus Intra-Host Populations Are Characterized by Numerous High Frequency Variants.
Auteurs : Monica K. Borucki [États-Unis] ; Victoria Lao [États-Unis] ; Mona Hwang [États-Unis] ; Shea Gardner [États-Unis] ; Danielle Adney [États-Unis] ; Vincent Munster [États-Unis] ; Richard Bowen [États-Unis] ; Jonathan E. Allen [États-Unis]Source :
- PloS one [ 1932-6203 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient, Infections à coronavirus.
- pathogénicité : Coronavirus du syndrome respiratoire du Moyen-Orient.
- transmission : Animaux, Cellules Vero, Chameaux, Humains, Infections à coronavirus, Mutation.
English descriptors
- KwdEn :
- MESH :
- genetics : Coronavirus Infections, Middle East Respiratory Syndrome Coronavirus.
- pathogenicity : Middle East Respiratory Syndrome Coronavirus.
- transmission : Coronavirus Infections.
- Animals, Camelus, Chlorocebus aethiops, Humans, Mutation, Vero Cells.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging human pathogen related to SARS virus. In vitro studies indicate this virus may have a broad host range suggesting an increased pandemic potential. Genetic and epidemiological evidence indicate camels serve as a reservoir for MERS virus but the mechanism of cross species transmission is unclear and many questions remain regarding the susceptibility of humans to infection. Deep sequencing data was obtained from the nasal samples of three camels that had been experimentally infected with a human MERS-CoV isolate. A majority of the genome was covered and average coverage was greater than 12,000x depth. Although only 5 mutations were detected in the consensus sequences, 473 intrahost single nucleotide variants were identified. Many of these variants were present at high frequencies and could potentially influence viral phenotype and the sensitivity of detection assays that target these regions for primer or probe binding.
DOI: 10.1371/journal.pone.0146251
PubMed: 26790002
Affiliations:
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Borucki</name><affiliation wicri:level="2"><nlm:affiliation>Lawrence Livermore National Laboratory, Livermore, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Lawrence Livermore National Laboratory, Livermore, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Lao, Victoria" sort="Lao, Victoria" uniqKey="Lao V" first="Victoria" last="Lao">Victoria Lao</name><affiliation wicri:level="2"><nlm:affiliation>Lawrence Livermore National Laboratory, Livermore, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Lawrence Livermore National Laboratory, Livermore, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Hwang, Mona" sort="Hwang, Mona" uniqKey="Hwang M" first="Mona" last="Hwang">Mona Hwang</name><affiliation wicri:level="2"><nlm:affiliation>Lawrence Livermore National Laboratory, Livermore, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Lawrence Livermore National Laboratory, Livermore, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Gardner, Shea" sort="Gardner, Shea" uniqKey="Gardner S" first="Shea" last="Gardner">Shea Gardner</name><affiliation wicri:level="2"><nlm:affiliation>Lawrence Livermore National Laboratory, Livermore, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Lawrence Livermore National Laboratory, Livermore, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Adney, Danielle" sort="Adney, Danielle" uniqKey="Adney D" first="Danielle" last="Adney">Danielle Adney</name><affiliation wicri:level="2"><nlm:affiliation>Colorado State University, Fort Collins, Colorado, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Colorado State University, Fort Collins, Colorado</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Munster, Vincent" sort="Munster, Vincent" uniqKey="Munster V" first="Vincent" last="Munster">Vincent Munster</name><affiliation wicri:level="2"><nlm:affiliation>National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Bowen, Richard" sort="Bowen, Richard" uniqKey="Bowen R" first="Richard" last="Bowen">Richard Bowen</name><affiliation wicri:level="2"><nlm:affiliation>Colorado State University, Fort Collins, Colorado, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Colorado State University, Fort Collins, Colorado</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Allen, Jonathan E" sort="Allen, Jonathan E" uniqKey="Allen J" first="Jonathan E" last="Allen">Jonathan E. Allen</name><affiliation wicri:level="2"><nlm:affiliation>Lawrence Livermore National Laboratory, Livermore, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Lawrence Livermore National Laboratory, Livermore, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Camelus</term><term>Chlorocebus aethiops</term><term>Coronavirus Infections (genetics)</term><term>Coronavirus Infections (transmission)</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (pathogenicity)</term><term>Mutation</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Chameaux</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité)</term><term>Humains</term><term>Infections à coronavirus (génétique)</term><term>Infections à coronavirus (transmission)</term><term>Mutation</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus Infections</term><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Camelus</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Mutation</term><term>Vero Cells</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Chameaux</term><term>Humains</term><term>Infections à coronavirus</term><term>Mutation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging human pathogen related to SARS virus. In vitro studies indicate this virus may have a broad host range suggesting an increased pandemic potential. Genetic and epidemiological evidence indicate camels serve as a reservoir for MERS virus but the mechanism of cross species transmission is unclear and many questions remain regarding the susceptibility of humans to infection. Deep sequencing data was obtained from the nasal samples of three camels that had been experimentally infected with a human MERS-CoV isolate. A majority of the genome was covered and average coverage was greater than 12,000x depth. Although only 5 mutations were detected in the consensus sequences, 473 intrahost single nucleotide variants were identified. Many of these variants were present at high frequencies and could potentially influence viral phenotype and the sensitivity of detection assays that target these regions for primer or probe binding. </div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Colorado</li><li>Montana</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Borucki, Monica K" sort="Borucki, Monica K" uniqKey="Borucki M" first="Monica K" last="Borucki">Monica K. Borucki</name></region><name sortKey="Adney, Danielle" sort="Adney, Danielle" uniqKey="Adney D" first="Danielle" last="Adney">Danielle Adney</name><name sortKey="Allen, Jonathan E" sort="Allen, Jonathan E" uniqKey="Allen J" first="Jonathan E" last="Allen">Jonathan E. Allen</name><name sortKey="Bowen, Richard" sort="Bowen, Richard" uniqKey="Bowen R" first="Richard" last="Bowen">Richard Bowen</name><name sortKey="Gardner, Shea" sort="Gardner, Shea" uniqKey="Gardner S" first="Shea" last="Gardner">Shea Gardner</name><name sortKey="Hwang, Mona" sort="Hwang, Mona" uniqKey="Hwang M" first="Mona" last="Hwang">Mona Hwang</name><name sortKey="Lao, Victoria" sort="Lao, Victoria" uniqKey="Lao V" first="Victoria" last="Lao">Victoria Lao</name><name sortKey="Munster, Vincent" sort="Munster, Vincent" uniqKey="Munster V" first="Vincent" last="Munster">Vincent Munster</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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